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ExactID® Help & Troubleshooting

Help Guide

 

Task 1: Select FASTQ Data Files for Analysis

1. Select Import FASTQ File from the Home tab. A file browser window will pop up.

 
ExactID screenshot

2. Browse to the folder where the FASTQ files are located. Select Open.


Task 2: Select Files and Enter Metadata

1. Check the box beside the files you wish to analyze. Enter metadata for SampleID, Analyst and Sample Type for selected files. Once the metadata is entered, the Finish button will activate.

 
ExactID screenshot

2.  FASTQ files imported into ExactID will appear under a file-tree in the Results Explorer Window. All files imported on any given day will be grouped within a tree node. Nodes can be expanded and contracted by clicking on + and – next to the node icons.    


Task 3: Analyze STR Panels

1. In the Results Explorer window, expand subdirectories to locate the FASTQ file of interest. Right click on the desired file and from the popup menu select one of either STR analysis method: Run STR or Run STR (Set Analytical threshold). The first option uses a previously established Analytical Threshold (AT) pre-set by an Administrative User. The second option allows the user to set the AT for the current analysis. 

2.  A popup menu will appear with a dropdown list. Select the desired forensic kit from the dropdown list. The administrator can change kits by changing configuration files. This menu may not appear if there is only one SNP kit in the configuration file.

 
ExactID screenshot


3.  The results file will appear below the FASTQ file icon in the Results Explorer Window. The file can be deleted or renamed by right-clicking on it.


ExactID screenshot


4. Double click on the results file to present results in the Display Window.


ExactID screenshot

5. Instrument responses above the AT will show in the Display Window. The analyst can select instrument responses for inclusion in the genotype by checking the box beside the responses.

6. Select the Called Alleles tab to observe graphs for alleles selected in the STR analysis window.

 
ExactID screenshot


7. Select the Visualization tab to observe all instrument responses above AT for each locus. True alleles and stutter will be observable.


ExactID screenshot


Task 4: Analyze SNP Panels

1. In the Results Explorer window, expand subdirectories to locate the FASTQ file of interest. Right click on the desired file and from the popup menu select Run SNP Analyze.

 
ExactID screenshot


2. A popup menu will appear with a dropdown list. Select the desired forensic kit from the dropdown list.  The administrator can change kits by changing configuration files. This menu may not appear if there is only one SNP kit in the configuration file.

3. The results file will appear below the FASTQ file icon in the Results Explorer Window. The file can be deleted or renamed by right-clicking on it.

4. Double click on the results file to present results in the Display Window.

5.  Instrument responses above the AT will show in the Display Window. Both the AT and Read Abundance Ratio values are user-configurable.

6. Select the SNP Results tab to observe stacked bar charts graphs displaying heterozygote balance for SNP loci.


ExactID screenshot
 

7.  Select the SNP Abundances tab to display a kopiagram showing all instrument responses above AT for each locus.


ExactID screenshot

 

Task 5: Report Results

ExactID produces three report outputs. A PDF-formatted report, a .sef file for evidence preservation, and a .csv file for transfer of data to other data systems. Prior to exporting data or reports, select the Save Changes button.

  • The PDF-formatted report presents the selected genotypes in both tabular and graphical format.

  • The .sef file preserves all instrument responses above AT, along with user-supplied metadata for evidence preservation. The .sef file is a human-readable summary of the raw data to be used in second reviews.

  • The .csv file provides essential genotype data for databasing, or for display in Excel or similar software.

 

Troubleshooting FAQ

After installing ExactID and entering the license key, the application will open but I can't see the window (it won't maximize).
This can happen the first time that ExactID is run after the license key is entered and validated. To restore the ExactID display window, click on the application in the task bar, and then simultaneously hold down the Windows logo key and the Up Arrow key.

On occasion ExactID will open without loading some of all of its buttons.

Try closing and reopening ExactID to correct this issue.

If you have closed or undocked the results explorer window you can restore it by following this procedure.
Once you have docked the Results Explorer window, you can restore it by clicking the maximize icon. 

If you close the Results Explorer window, you can restore it by clicking the ExactID™ icon at the far upper left of the display, then selecting Window->Results Explorer. 

You can also drag the Results Explorer window completely outside the ExactID™ display, in which case it becomes a new window. You can restore its original configuration by clicking the ExactID™ icon->Window->Reset Windows.  

If a file is deleted from your database using a method other than right-clicking on the file name within ExactID you may see a red exclamation point warning icon beside the file name in the results explorer window.
Currently there is no function that can clear the removed file name or the warning icon if a file is removed in this manner.  Be sure to always delete any files from within the ExactID software  by right clicking on the file name and selecting Delete.